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Abstract
This study presents a novel and efficient high-performance liquid chromatography (HPLC) method for quantifying paracetamol in pharmaceutical products. The method utilizes a custom-fabricated monolithic column composed of poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate-co-acrylic acid). The monolithic structure was synthesized in situ within a borosilicate glass capillary through ultraviolet (UV) polymerization at 365 nm. The resulting polymer matrix exhibited high porosity, which significantly enhanced surface area, permeability, and mass transfer efficiency—factors that contributed to improved chromatographic resolution and reduced analysis time. Chromatographic separation was achieved using a mobile phase consisting of acetonitrile and water (90:10, v/v) at a flow rate of 0.3 mL/min, with detection performed at a wavelength of 296 nm. The developed method demonstrated excellent linearity over a concentration range of 3 to 100 µg/mL, with a correlation coefficient (R²) of 0.9998. The calculated limits of detection (LOD) and quantification (LOQ) were 0.817 µg/mL and 2.696 µg/mL, respectively.