In vitro Antioxidant and Anti-Inflammatory Effects of the Hydro-Methanolic Extract of Phlomis crinita from North Algeria
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Keywords

Phlomis crinita, Phenolic compounds, Inflammation, Stress.

How to Cite

Boutennoun, H. ., Boussouf, L. ., Balli, N. ., Makhlouf, L. ., Madani, K. ., Desdous, N. ., Bouchefifa, F. ., Boudehane, E. ., & Al-qaoud, K. . (2023). In vitro Antioxidant and Anti-Inflammatory Effects of the Hydro-Methanolic Extract of Phlomis crinita from North Algeria . Jordan Journal of Chemistry (JJC), 18(2), 77-85. Retrieved from https://jjc.yu.edu.jo/index.php/jjc/article/view/645

Abstract

Phlomis crinita is a medicinal plant commonly used in Algerian folk medicine as a remedy for ulcers, haemorrhoids and healing of wounds. The current study aimed to evaluate the anti-inflammatory and antioxidant effects of this plant and to provide a scientific basis for its use. Protein denaturation and membrane stabilization assays were used to assess the anti-inflammatory action in vitro. DPPH free radical-scavenging and reducing power tests were used to determine the antioxidant activity. The plant methanol extract exhibited potential anti-inflammatory activity, demonstrating its ability to prevent protein denaturation and stabilize the membrane of human red blood cells. It had IC50 values of 143.64 ± 2.94 and 119.63 ± 0.80 µg/mL, respectively, compared to diclofenac sodium (IC50 value of 60.60 ± 2.55 and 53.47 ± 0.93 µg/mL, respectively). P. crinita showed strong antioxidant DPPH scavenging activity (IC50 = 82.07±0.37 μg/mL) compared to ascorbic acid (62.48±0.56) and exhibited a strong reducing potential. The results suggested that the plant might be a potential candidate for oxidative stress prevention and inflammatory disease treatment.

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