Abstract: Molybdenum disulfide, MoS2, is commonly used as a substrate in supercapacitors and other energy production systems, but it is of limited utility in pharmaceutical analysis. In this article, a molybdenum disulfide composite was prepared, and the electro-activity of MoS2 toward the oxidation of omeprazole (OMZ) was investigated. The molybdenum disulfide content of the prepared material was quantified by thermal gravimetric analysis (TGA), its crystallinity revealed by X-ray diffraction (XRD, and its topographic features were probed by scanning electron microscopy (SEM). Glassy carbon electrode was modified by MoS2, and the electrochemical activity of the modified electrode toward omeprazole oxidation was investigated in a phosphate buffer solution (pH 7.00) using differential pulse voltammetry (DPV). The well-resolved anodic peak at ca. 0.85 V vs Ag/AgCl was reported at the modified and bare glassy carbon electrodes. The impact of the modification with MoS2 was noticed as a 2.3-fold enhancement of the OMZ oxidation current. Under the applied experimental conditions and utilising the modified electrode, a linear response was obtained in the range of 15.8–500. μM with a limit of detection equal to 4.74 μM and a correlation coefficient of 0.989. The modified electrode was also employed to detect omeprazole in its commercial pharmaceutical formulations. Percent recovery values of 84.0 to 100.% were reported.