Mixed ligand complexes of the type [ML1L2(H2O)n]Cl (where M= CoII, NiΙΙ and CuII, L1 =urea and L2 =histidine) have been synthesized by the reactions of anhydrous metal chlorides with the two ligands in 1:1:1 molar ratios. The resulting complexes have been characterized by elemental analysis, molar conductivity measurement, IR, mass, electronic spectra, magnetic susceptibility measurements, in addition to thermal analysis. The molar conductance values ranging from 103.22 to 128.94 cm2mol-1Ω-1 show that these complexes are of electrolytic nature (in DMSO solvent) and clearly indicate the non - coordination of the anion chloride with the metal ions. The spectral data show that the complexes with NiΙΙ and CuΙΙ are of octahedral structure, whereas CoΙΙ is square-planar. Unfortunately, the biological screening of these complexes showed no activity against the four studied strains, i.e, Staphylococcus aureus (TCC 29737), Bacillus subtilis(ATCC 6633),Escherichia coli (ATCC 11303) and Pseudomonas aeruginosa (ATCC 25619).